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A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
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JoVE 신문 생물학
A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT
DOI:

07:10 min

June 12, 2020

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Chapters

  • 00:04Introduction
  • 01:10Tissue Sectioning
  • 03:38Sample Coring
  • 05:29Results: SRμCT Reconstructed Slices of the Anterior Aspect of the Left Femur from Two Individuals
  • 06:37Conclusion

Summary

자동 번역

We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SRµCT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

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