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使用延时荧光显微镜对细菌捕食者 Bdellovibrio 细菌的生命周期进行活细胞成像

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Live-Cell Imaging of the Life Cycle of Bacterial Predator Bdellovibrio bacteriovorus using Time-Lapse Fluorescence Microscopy

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使用延时荧光显微镜对细菌捕食者 Bdellovibrio 细菌的生命周期进行活细胞成像

DOI:

10.3791/61105-v

•

08:56 min

•

May 08, 2020

•

Łukasz Makowski, Damian Trojanowski, Jolanta Zakrzewska-Czerwińska

¹Department of Molecular Microbiology, Faculty of Biotechnology,University of Wroclaw

Chapters

00:04Introduction
00:34B. bacteriovorus Lysate Preparation
01:47Host Cell Preparation
02:32Time-Lapse Fluorescence Microscopy Layout Preparation
03:36Time-Lapse Fluorescence Microscopy
05:30Time-Lapse Image Processing and Movie Generation
07:11Results: Representative B. bacteriovorus Life Cycle Captured by Time-Lapse Fluorescence
08:16Conclusion

Summary

자동 번역

此处介绍的是一个协议，它描述了使用延时荧光显微镜与 agarose 垫和细胞成像盘相结合的掠夺性细菌 Bdellovibrio 细菌的完整生命周期的监测。

Tags

Keywords: Live-cell Imaging Bdellovibrio Bacteriovorus Time-lapse Fluorescence Microscopy Bacterial Predator Life Cycle Prey Cells Agarose Pad Calcium HEPES Buffer Optical Density Centrifugation Fluorescence Microscopy Prey Lysis Multi-attack Phase

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