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Chapters
- 00:05Introduction
- 01:00Microscope Setup
- 01:50Image Acquisition
- 02:502D Image Analysis
- 04:22Statistical Analysis
- 05:03Results: Representative Interspecies Segmentation and Variability Analyses
- 06:46Conclusion
Here, a protocol is presented for optically extracting and cataloging innate cellular fluorescence signatures (i.e., cellular autofluorescence) from every individual live cell distributed in a three-dimensional space. This method is suitable for studying the innate fluorescence signature of diverse biological systems at a single-cell resolution, including cells from bacteria, fungi, yeasts, plants, and animals.
