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Journal / Developmental Biology / 从鼠心心室自由壁分离内膜细胞和冠状内皮细胞…
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Developmental Biology

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JoVE Journal Developmental Biology
从鼠心心室自由壁分离内膜细胞和冠状内皮细胞
 
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从鼠心心室自由壁分离内膜细胞和冠状内皮细胞

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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我们提出了一种方案，通过消化缓冲液中的连续组织消化、循环离心循环的细胞采集以及使用抗大鼠CD31微珠进行细胞纯化，从大鼠心脏分离内皮和冠状内皮细胞。

Tags

发育生物学， 第158期， 内膜， 冠状动脉内膜， 隔离， 心脏， 大鼠， CD31
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