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Chapters
- 00:05Introduction
- 00:30Non‐Stressful Embryo Synchronization
- 02:06Common Phenotypic Assays: Animal Cultivation, Embryonic Lethality, and Paralysis
- 03:30Protein Knockdown Validation
- 05:02Results: Representative Tissue‐Specific Chaperone Interaction Screening
- 06:26Conclusion
To study chaperone-chaperone and chaperone-substrate interactions, we perform synthetic interaction screens in Caenorhabditis elegans using RNA interference in combination with mild mutations or over-expression of chaperones and monitor tissue-specific protein dysfunction at the organismal level.
