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1Department of Radiobiology, Faculty of Military Health Sciences in Hradec Kralove,University of Defence, 2Department of Clinical Biochemistry and Diagnostics,University Hospital, 3Department of Oncology, Thomayer Hospital,Charles University, 4Department of Oncology and Radiotherapy, Faculty of Medicine,Charles University
Chapters
- 00:04Introduction
- 00:40Cell Culture for Immunocytochemistry Staining
- 03:10Immunofluorescent Staining of Primary Cilia In Vitro
- 06:11Results: Fluorescent Imaging of Primary Cilia in Fibroblasts
- 07:26Conclusion
Primary cilia are extracellular structures associated with the centriole. Primary cilia detection by immunofluorescent staining is a relatively simple procedure that results in extremely high-quality images. In this protocol, fibroblasts expressing primary cilia were fixed, immunostained, and imaged in a fluorescent or confocal microscope.
