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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
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A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

DOI:
10.3791/61160-v

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06:50 min

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May 29, 2020

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, , , ,
1Barbara Davis Center for Diabetes,University of Colorado School of Medicine, 2Department of Endocrinology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine,Chinese Academy of Medical Sciences, 3Department of endocrinology, Guangzhou First People’s Hospital, School of Medicine,South China University of Technology

Chapters

  • 00:04Introduction
  • 00:50Mixed Linker-Coupled Antigen Solution
  • 02:26Serum Sample Incubation with Labeled Antigen
  • 03:30Multiplex Plate Preparation
  • 03:57Serum/Antigen Incubate Transfer
  • 04:58Results: Representative Autoantibody Level Evaluation
  • 06:03Conclusion

Summary

자동 번역

자동 번역

We model a simple multiplexed ECL assay that combines 7 autoantibody assays together. The assay is capable of screening for T1D and multiple other autoimmune diseases, simultaneously, including celiac disease, autoimmune thyroid disease, and autoimmune polyglandular syndrome 1.

Tags

Electrochemiluminescence AssayType 1 DiabetesAutoimmune DiseasesHigh-throughputMultiplexAntigen LabelingSerum IncubationAutoantibody Detection

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