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Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions
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Chapters
- 00:04Introduction
- 00:57Zebrafish Larva Hindbrain Ventricle Microinjection
- 04:21Establishment of Injected and Viable Spore Numbers
- 05:22Drug Treatment of Larvae and Survival Analysis
- 05:53Zebrafish Wounding and Entrapment Device for Growth and Imaging
- 07:23Results: Infection Outcome of Aspergillus Spores Microinjected into Zebrafish Larvae
- 09:07Conclusion
This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit plating.
