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Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
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Chapters
- 00:04Introduction
- 01:00Extracellular DNA (ecDNA) Staining
- 03:50Staining Analysis
- 07:18Results: Representative ecDNA Identification and Quantification
- 08:24Conclusion
Extracellular DNA (ecDNA) released during cell death is proinflammatory and contributes to inflammation. Measurement of ecDNA at the site of injury can determine the efficacy of therapeutic treatment in the target organ. This protocol describes the use of a machine learning tool to automate measurement of ecDNA in kidney tissue.
