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斑马鱼中具有功能化荧光硅纳米粒子的异种移植人类癌细胞的Vivo靶向
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JoVE 신문 암 연구학
In Vivo Targeting of Xenografted Human Cancer Cells with Functionalized Fluorescent Silica Nanoparticles in Zebrafish
DOI:

10:26 min

May 08, 2020

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Chapters

  • 00:04Introduction
  • 00:54Generation of Casper Zebrafish Embryos
  • 02:33Preparing Materials for Cancer Cell Transplantation
  • 03:34Preparing Cancer Cells for Transplantation
  • 05:20Transplantation of Cancer Cells
  • 06:54Injection of Nanoparticles or Vehicle
  • 07:40Imaging and Tracking of Nanoparticles and Cancer Cells
  • 08:45Results: Colocalization of Cancer Cells and Nanoparticles
  • 09:47Conclusion

Summary

자동 번역

这里描述的方法是利用斑马鱼胚胎研究功能化纳米粒子在体内瞄准人类癌细胞的能力。此方法允许评估和选择最佳纳米粒子,供将来在大型动物和临床试验中进行测试。

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