Journal
/
의학
/
Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber
JoVE 신문
의학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 의학
Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

DOI:
10.3791/61234-v

•

09:07 min

•

June 11, 2020

•

, ,
1Department of Experimental Medical Science,Lund University, 2Lund University Diabetes Centre

Chapters

  • 00:04Introduction
  • 01:08Transplantation Procedure
  • 02:45Image Analysis
  • 06:58Results: Image Segmentation of a Human Islet Grafted in the ACE of a NOD.ROSA-tomato.Rag2-/- Recipient Mouse
  • 08:17Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to continuously monitor the dynamics of the human pancreatic islet engraftment process and the contributing host versus donor cells. This is accomplished by transplanting human islets into the anterior chamber of the eye (ACE) of an NOD.(Cg)-Gt(ROSA)26Sortm4-Rag2-/-mouse recipient followed by repeated 2-photon imaging.

Tags

In Vivo ImagingPancreatic Islet GraftingAnterior Eye ChamberBeta Cell Replacement TherapyIslet TransplantationTwo-photon MicroscopyIslet Function And ViabilityDiabetic ComplicationsReal-time Imaging

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61234?article_type=v

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
check_url/kr/61234?article_type=v

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
check_url/kr/61234?article_type=v

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
check_url/kr/61234?article_type=v

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
check_url/kr/61234?article_type=v

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy
check_url/kr/61234?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/kr/61234?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/kr/61234?article_type=v

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
check_url/kr/61234?article_type=v

Laser Doppler: A Tool for Measuring Pancreatic Islet Microvascular Vasomotion In Vivo
check_url/kr/61234?article_type=v

Human Fetal Blood Flow Quantification with Magnetic Resonance Imaging and Motion Compensation

Read Article