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Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
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Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization
DOI:

11:29 min

April 28, 2021

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Chapters

  • 00:00Introduction
  • 00:35Larval Brain Dissection
  • 02:23Adult Brain Dissection
  • 04:03Mounting Larval Brains
  • 06:06Mounting Adult Brains
  • 08:02Representative Images of Larval and Adult Optic Lobes
  • 10:47Conclusion

Summary

자동 번역

This protocol describes three steps to prepare larval and adult Drosophila optic lobes for imaging: 1) brain dissections, 2) immunohistochemistry and 3) mounting. Emphasis is placed on step 3, as distinct mounting orientations are required to visualize specific optic lobe structures. 

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