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Cultivar neuronas simpáticas de ratas de Ganglia cervical superior embrionario para análisis morfológico y proteómico
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JoVE 신문 신경과학
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
DOI:

14:51 min

September 27, 2020

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Chapters

  • 00:00Introduction
  • 00:09Preparation of Media for Dissection and for Culturing Neurons
  • 00:42Preparation of Plates for Culturing Neurons
  • 01:56Dissection Set-up
  • 02:36Embryo Removal and Preparation
  • 04:17Isolation of the SCG from the Embryo
  • 06:52Post-processing of SCG
  • 11:00Maintenance of the Cultured SCG Neurons and Treatments
  • 13:02Representative Results- Immunostaining
  • 14:00Representative Results- Proteomic Analysis
  • 14:29Conclusion

Summary

자동 번역

Este artículo describe el aislamiento y el cultivo de las neuronas simpatizantes de ratas embrionarias de los ganglios cervicales superiores. También proporciona protocolos detallados para la tinción inmunocitoquímica y para la preparación de extractos neuronales para el análisis espectrométrico de masas.

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