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JoVE Journal Biology
Proteolytisk nedbrudt alginathydrgels og hydrofobe mikrobioreaktorer til Porcine Oocyt Indkapsling
 
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Proteolytisk nedbrudt alginathydrgels og hydrofobe mikrobioreaktorer til Porcine Oocyt Indkapsling

Article DOI: 10.3791/61325-v • 07:45 min • July 30th, 2020
July 30th, 2020 •
1, 2, 3, 1
1Department of Endocrinology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow, 2Department of Histology, Jagiellonian University Medical College, 3Department of Experimental Hematology, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Krakow

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Præsenteret her er to protokoller for indkapsling af svin oocytter i 3D-kultur betingelser. I den første er cumulus-oocyt komplekser (P-piller) indkapslet i fibrin-alginat perler. I den anden, de er omgivet af fluorerede ethylen propylen pulverpartikler (mikrobioreaktorer). Begge systemer sikrer optimale betingelser for at opretholde deres 3D-organisation.

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Denne måned i JoVE cumulus-oocyt komplekser 3D indkapsling alginat mikrobioreaktorer FEP
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