Journal
/
면역학 및 감염병학
/
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

DOI:
10.3791/61359-v

•

09:00 min

•

June 24, 2020

•

, , ,
1Department of Microbiology, Immunology, and Cell Biology,West Virginia University, 2Department of Neuroscience,West Virginia University, 3Rockefeller Neuroscience Institute,West Virginia University

Chapters

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Summary

자동 번역

자동 번역

This protocol describes the use of flow cytometry to identify the changes in immune cell composition, cytokine profile, and chemokine profile in the pulmonary environment following transient middle cerebral artery occlusion, a murine model of ischemic stroke.

Tags

Immune CellsProinflammatory MediatorsPulmonary EnvironmentIschemic StrokeFlow Cytometric AnalysisLung Immune Cell TypesCytokinesChemokinesWhole Body Transcardial PerfusionMultiplex Bead ArraysHomogenization BufferLung Cell Medium

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

Seven Steps to Stellate Cells

Isolation of Functional Cardiac Immune Cells

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation

Right Ventricular Systolic Pressure Measurements in Combination with Harvest of Lung and Immune Tissue Samples in Mice

Isolation of Adipose Tissue Immune Cells

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Read Article