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Chapters
- 00:04Introduction
- 00:39Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest
- 03:15Insulin-Stimulated Glucose Uptake
- 04:18Contraction-Stimulated Glucose Uptake
- 04:57Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination
- 05:58Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis
- 07:22Conclusion
Intact regulation of muscle glucose uptake is important for maintaining whole body glucose homeostasis. This protocol presents assessment of insulin- and contraction-stimulated glucose uptake in isolated and incubated mature skeletal muscle when delineating the impact of various physiological interventions on whole body glucose metabolism.
