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Modellering hepatit B virusinfektion i icke-lever 293T-NE-3NRs Celler
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
DOI:

09:02 min

June 05, 2020

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Chapters

  • 00:04Introduction
  • 01:36HepG2.2.15 Cell Culture
  • 03:04Detection of HBV DNA in Supernatant Concentrate
  • 04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
  • 05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
  • 06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
  • 07:47Conclusion

Summary

자동 번역

Detta manuskript beskriver ett detaljerat protokoll för hepatit B-virus (HBV) infektion i nya konstruerade 293T celler (293T-NE-3NRs, uttrycker mänskliga NTCP, HNF4α, RXRα och PPARα) och traditionella leverceller (HepG2-NE, uttrycker mänskliga NTCP).

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