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Hepatik Olmayan 293T-NE-3NR Hücrelerinde Hepatit B Virüs Enfeksiyonunun Modelilmesi
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute,General Autority – City of Scientific Researches and Technological Applications
Chapters
- 00:04Introduction
- 01:36HepG2.2.15 Cell Culture
- 03:04Detection of HBV DNA in Supernatant Concentrate
- 04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
- 05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
- 06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
- 07:47Conclusion
