Journal
/
신경과학
/
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
JoVE 신문
신경과학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Please note that all translations are automatically generated. Click here for the English version.

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

DOI:
10.3791/61434-v

•

14:02 min

•

October 31, 2020

•

, , , , , , , ,
1Department of Molecular Biochemistry and Pharmacology,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience,Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute,Istituto di Ricerche Farmacologiche Mario Negri IRCCS

Chapters

  • 00:00Introduction
  • 01:04Primary Cultures
  • 01:48Immunofluorescence Staining
  • 03:32SIM Microscopy
  • 04:01Microscope Calibration
  • 07:11Acquisition
  • 09:24Post-production: Image Reconstruction
  • 11:24Colocalization with Profile Analysis
  • 12:05Quantification of Pearson’s and Mander’s
  • 12:27Results
  • 13:16Conclusions

Summary

자동 번역

자동 번역

Dit protocol laat zien hoe je microscopie met superresolutie gebruiken om eiwitcolokalisatie te bestuderen in primaire neuronale culturen.

Tags

Super-resolution ImagingCo-localizationPrimary NeuronsSynaptic MarkersStructured Illumination Microscopy (SIM)Neurodegenerative DisordersHippocampal NeuronsFixationImmunostainingMounting SolutionCoverglass

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61434?article_type=v

Laser capture microdissectie van Drosophila Perifere neuronen
check_url/kr/61434?article_type=v

Vibrodissociation van de neuronen van knaagdieren Brain Slices synaptische transmissie en Image presynaptische Terminals Study
check_url/kr/61434?article_type=v

Laterale diffusie en Exocytose van membraaneiwitten in gekweekte zenuwcellen gemeten met behulp van Fluorescence Recovery en fluorescentie-verlies fotobleken
check_url/kr/61434?article_type=v

Imaging pHluorin-tagged Receptor invoegen in op het plasmamembraan in primaire gekweekte muis neuronen
check_url/kr/61434?article_type=v

In vivo twee-foton beeldvorming van ervaring-afhankelijke moleculaire veranderingen in corticale neuronen
check_url/kr/61434?article_type=v

Super-resolution Imaging van Neuronal Dense-core Blaasjes
check_url/kr/61434?article_type=v

Real-time beeldvorming van Axonale Vervoer van Quantum Dot-label BDNF in primaire neuronen
check_url/kr/61434?article_type=v

Meten van Synaptic vesikel endocytose in gekweekte Hippocampal neuronen
check_url/kr/61434?article_type=v

Modelleren van neuronale dood en degeneratie in muis primaire cerebellaire submodule neuronen
check_url/kr/61434?article_type=v

Een optische Assay voor Synaptic vesikel Recycling in gekweekte neuronen Overexpressing presynaptische eiwitten

Read Article