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从成人人类脑组织获取人类微胶质

JoVE 신문
신경과학
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JoVE 신문 신경과학

Obtaining Human Microglia from Adult Human Brain Tissue

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从成人人类脑组织获取人类微胶质

DOI:

10.3791/61438-v

•

09:41 min

•

August 30, 2020

•

Ishan Agrawal, Shivanjali Saxena, Preethika Nair, Deepak Jha, Sushmita Jha

¹Department of Bioscience and Bioengineering,Indian Institute of Technology Jodhpur, ²Department of Neurosurgery,All India Institute of Medical Sciences Jodhpur

Chapters

00:00Introduction
01:06Tissue Acquisition and Processing: Day 0
05:35Cell Culture: Day 2
07:59Cell Culture: Day 4
08:26Results: Representative Images of Adult Human Microglia
09:15Conclusion

Summary

자동 번역

该协议是一种高效、经济、稳健的方法，将原发性微胶质与活的、成人的、人类脑组织隔离在一起。分离的原人类微胶质可以作为研究平衡和疾病细胞过程的工具。

Tags

Keywords: Human Microglia Primary Microglia Cells Immunocytochemistry Cell Isolation Trypsin-EDTA Centrifugation Cell Culture

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