Journal
/
발생학
/
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells

DOI:
10.3791/61446-v

•

08:47 min

•

May 15, 2020

•

, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Chapters

  • 00:04Introduction
  • 00:58EB, NPC, and Neuron Differentiation
  • 03:00Fixation and Embedding
  • 04:24Immunofluorescence
  • 05:45Results: E14 Differentiation into EBs, NPCs, and Neurons
  • 07:41Conclusion

Summary

자동 번역

자동 번역

We describe the procedure for the in vitro differentiation of mouse embryonic stem cells into neuronal cells using the hanging drop method. Furthermore, we perform a comprehensive phenotypic analysis through RT-qPCR, immunofluorescence, RNA-seq, and flow cytometry.

Tags

Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Neural Differentiation of Mouse Embryonic Stem Cells in Serum-free Monolayer Culture
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
check_url/kr/61446?article_type=v&slug=differentiation-characterization-neural-progenitors-neurons-from

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Read Article