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Chapters
- 00:04Introduction
- 01:03Nuclear Extraction and Cell Fixation
- 02:04Immunofluorescence Staining
- 03:27Image Acquisition
- 04:52Data Analysis
- 05:30Results: Protein-protein Interaction at Sites of DNA Damage
- 07:03Conclusion
Following DNA damage, human cells activate essential repair pathways to restore the integrity of their genome. Here, we describe the method of indirect immunofluorescence as a means to detect DNA repair proteins, analyze their spatial and temporal recruitment, and help interrogate protein-protein interaction at the sites of DNA damage.
