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1Cardiovascular Branch, National Heart, Lung and Blood Institute,National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa,Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute,National Institutes of Health
Chapters
- 00:04Introduction
- 00:51NK Cells Isolation from Peripheral Blood
- 03:33Extracellular Flux Assay
- 06:40Results: NK Cell Function and Metabolism
- 08:36Conclusion
In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.
