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使用洗涤剂和酶无酶方法从整个组织进行核分离
JoVE 신문
생물학
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JoVE 신문 생물학
Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
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使用洗涤剂和酶无酶方法从整个组织进行核分离

DOI:
10.3791/61471-v

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07:00 min

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June 24, 2020

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, ,
1IRIBHM,Université Libre de Bruxelles (ULB), 2Medecine Faculty,Université Libre de Bruxelles (ULB)

Chapters

  • 00:04Introduction
  • 00:50Preparation for Tissue Dissection
  • 01:36Zebrafish Brain Dissection and Single Nuclei Isolation
  • 03:59FACS Based Nuclei Enrichment
  • 05:16Results: Single Nuclei Isolation and Enrichment
  • 06:04Conclusion

Summary

자동 번역

자동 번역

单核分离依赖于细胞膜的分离和基于洗涤剂的渗透，这些步骤需要优化，并且容易引入技术伪影。我们演示了一种洗涤剂和无酶协议，用于直接从整个组织中快速分离完整的核，产生适合单核RNA-seq（snRNA-Seq）或 ATAC-seq 的核。

Tags

Keywords: Nuclei IsolationSingle Nuclei SuspensionDetergent-freeEnzyme-freeHigh Throughput TranscriptomeEpigenome ProfilingCell LysisNuclei PurificationNuclei ResuspensionCentrifugation

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