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Extractie en kwantificering van oplosbare, radiogelabelde Inositol Polyfosfaten van verschillende plantensoorten met SAX-HPLC
JoVE 신문
생화학
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JoVE 신문 생화학
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
DOI:

09:01 min

June 26, 2020

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Chapters

  • 00:04Introduction
  • 00:50HPLC System Set Up
  • 01:37Plant Cultivation and Labeling with [3H]-myo-inositol
  • 03:02Extraction of Soluble InsPs
  • 04:31HPLC
  • 05:51Data Analysis
  • 06:45Results: InsP profiles of A. thaliana and L. japonicus
  • 08:15Conclusion

Summary

자동 번역

Hier beschrijven we sterke anion uitwisseling high-performance vloeibare chromatografie van [3H]-myo-inositol-gelabelde zaailingen die een zeer gevoelige methode is om inositol polyfosfaten in planten te detecteren en te kwantificeren.

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