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1Department of Physics,University of Illinois at Urbana-Champaign, 2Center for the Physics of Living Cells,University of Illinois at Urbana-Champaign, 3Carl R. Woese Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology,University of Illinois at Urbana-Champaign
Chapters
- 00:04Introduction
- 00:42Coverslip and Glass Slide Preparation
- 01:46Time-Course Experiment and Sample Fixation
- 03:18Cell Membrane Permeabilization
- 03:57Probe Hybridization
- 04:31Imaging
- 05:25Post-Hybridization Wash and Imaging Preparation
- 06:20Image Analysis
- 07:36Results: Representative mRNA Kinetic Probing Analysis
- 09:22Conclusion
