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Chapters
- 00:04Introduction
- 00:50Coating Bare Metal Surfaces with PEI-PDT
- 02:37Attaching Human PepCD47 to PEI-PDT Modified Surfaces
- 03:21Chandler Loop for Analyzing Cellular Attachment to Metal Surfaces
- 04:54Analyzing Cellular Attachment to Metal Surfaces using CFDA Dye
- 05:48Results: Anti-inflammatory Properties of PepCD47 Coated Metal Surfaces Exposed to Whole Blood and Isolated Monocytes
- 07:16Conclusion
Présenté ici est un protocole pour l’appending peptide CD47 (pepCD47) aux endonts métalliques utilisant la chimie de polybisphosphonate. La fonctionnalisation des enprothents métalliques à l’aide de pepCD47 empêche l’attachement et l’activation des cellules inflammatoires améliorant ainsi leur biocompatibilité.
