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Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches

DOI:
10.3791/61551-v

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09:35 min

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April 20, 2021

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, , ,
1Department of Biology,The City College of New York and The Graduate Center of The City University of New York, 2Department of Microbiology,Icahn School of Medicine at Mount Sinai

Chapters

  • 00:05Introduction
  • 01:03Dissection of Peyer’s Patches
  • 02:25Cell Isolation and Staining for FACS
  • 05:12JH4 Intron Sequence Amplification and Analysis
  • 07:39Results: Flow Cytometry and JH4 Sequence Analysis
  • 08:52Conclusion

Summary

자동 번역

자동 번역

Presented here is an assay to quantify somatic hypermutation within the immunoglobulin heavy chain gene locus using germinal center B cells from mouse Peyer’s patches.

Tags

Keywords: Somatic HypermutationJH4 IntronGerminal Center B CellsPeyer’s PatchesAntibody DiversificationMolecular BiologyPCRSanger SequencingCell Isolation

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