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从小鼠佩耶的补丁中对Germinal中心B细胞JH4因特龙的体细胞突变分析

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer’s Patches

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从小鼠佩耶的补丁中对Germinal中心B细胞JH4因特龙的体细胞突变分析

DOI:

10.3791/61551-v

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09:35 min

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April 20, 2021

•

Emily Sible, Simin Zheng, Jee Eun Choi, Bao Q. Vuong

¹Department of Biology,The City College of New York and The Graduate Center of The City University of New York, ²Department of Microbiology,Icahn School of Medicine at Mount Sinai

Chapters

00:05Introduction
01:03Dissection of Peyer’s Patches
02:25Cell Isolation and Staining for FACS
05:12JH4 Intron Sequence Amplification and Analysis
07:39Results: Flow Cytometry and JH4 Sequence Analysis
08:52Conclusion

Summary

자동 번역

这里介绍的是一个检测，以量化免疫球蛋白重链基因轨迹内的体细胞突变使用细菌中心B细胞从小鼠Peyer的补丁。

Tags

Keywords: Somatic Hypermutation JH4 Intron Germinal Center B Cells Peyer’s Patches Antibody Diversification Molecular Biology PCR Sanger Sequencing Cell Isolation

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