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1Center for Childhood Cancer and Blood Diseases,Abigail Wexner Research Institute at Nationwide Children’s Hospital, 2Molecular, Cellular, and Developmental Biology Program,The Ohio State University, 3Division of Pediatric Hematology/Oncology/Blood & Marrow Transplant,The Ohio State University, 4Department of Pediatrics,The Ohio State University
Chapters
- 00:04Introduction
- 00:55In Vitro Construct Panel Setup
- 02:07Cell Collection
- 02:47Construct Expression Validation and Correlative Phenotypic Assay Setup
- 03:50Alignment and Transcript Counting Pipeline
- 05:32Differential Expression
- 07:40Results: Representative Disordered Oncogenic Transcription Factor Structure-Function Relationship Mapping
- 09:31Conclusion
Iboende uorden domæner er vigtige for onkogen fusion transskription faktor funktion. For terapeutisk at målrette disse proteiner er det nødvendigt med en mere detaljeret forståelse af de reguleringsmekanismer, der anvendes af disse domæner. Her bruger vi transcriptomics til at kortlægge vigtige strukturelle træk ved det iboende uordnede EWS-domæne i Ewing sarkom.
