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Chapters
- 00:04Introduction
- 00:41Cannula Preparation
- 01:39Mouse Heart Extraction
- 02:33Septal Artery Preparation and Cannulation
- 03:47Preparation Stabilization
- 04:38Fluorescence-Tagged Wheat-Germ Agglutinin (WGA) Loading and Confocal Arteriole and Capillary Imaging
- 05:42Results: Representative Capillary and Arteriole Imaging and Luminal Pressure and Atrial Diameter Assessment
- 06:42Conclusion
Presented here is a protocol to study the coronary microcirculation in living murine heart tissue by ex vivo monitoring of the arterial perfusion pressure and flow that maintains the pressure, as well as vascular tree components including the capillary beds and pericytes, as the septal artery is cannulated and pressurized.
