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Chapters
- 00:04Introduction
- 00:41Cannula Preparation
- 01:39Mouse Heart Extraction
- 02:33Septal Artery Preparation and Cannulation
- 03:47Preparation Stabilization
- 04:38Fluorescence-Tagged Wheat-Germ Agglutinin (WGA) Loading and Confocal Arteriole and Capillary Imaging
- 05:42Results: Representative Capillary and Arteriole Imaging and Luminal Pressure and Atrial Diameter Assessment
- 06:42Conclusion
Hier wordt een protocol gepresenteerd om de coronaire microcirculatie in levend murinehartweefsel te bestuderen door ex vivo monitoring van de arteriële perfusiedruk en -stroom die de druk handhaaft, evenals vasculaire boomcomponenten, waaronder de capillaire bedden en pericyten, omdat de septale slagader wordt ingeblikt en onder druk staat.
