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鼠佩耶斑块中淋巴细胞动力学的微观观察

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Microscopic Observation of Lymphocyte Dynamics in Rat Peyer’s Patches

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鼠佩耶斑块中淋巴细胞动力学的微观观察

DOI:

10.3791/61568-v

•

06:31 min

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June 25, 2020

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Kazuhiko Shirakabe², Masaaki Higashiyama, Naoki Shibuya, Kazuki Horiuchi, Masayuki Saruta, Ryota Hokari

¹Department of internal medicine,National Defense Medical College, ²Division of Gastroenterology and Hepatology, Department of Internal Medicine,Jikei University School of Medicine

Chapters

00:04Introduction
00:42Collection and Separation of Lymphocytes
03:12Lymphocyte Labeling and Experimental Setup for Microvascular Studies
04:40Microcirculation of Lymphocytes
05:25Results: Microscopic Images of Peyer s Patches from Rats
05:56Conclusion

Summary

자동 번역

在这里，我们描述了一个精确的方法，收集胸管淋巴细胞和观察肠道热带淋巴细胞在老鼠Peyer的补丁的迁移3小时使用延时摄影。这项技术可以澄清淋巴细胞的动态在炎症条件下是如何受到影响的。

Tags

Lymphocyte Dynamics Peyer’s Patches In Vivo Observation Rat Cannulation Thoracic Duct Abdominal Surgery Anesthesia Heparin Duodenum Infusion Bollman’s Cage

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