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Akut nyreskade model induceret af Cisplatin hos voksne zebrafisk
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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Akut nyreskade model induceret af Cisplatin hos voksne zebrafisk

DOI:
10.3791/61575-v

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13:25 min

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May 15, 2021

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, , , , , ,
1Department of Immunology,University of São Paulo, 2Department of Medicine, Nephrology Division,Federal University of São Paulo

Chapters

  • 00:00Introduction
  • 01:48Cisplatin Injection
  • 03:41Tissue Processing for Flow Cytometry
  • 05:58Tissue Processing for TUNEL Assay
  • 07:46TUNEL Assay
  • 10:26Results
  • 12:29Conclusion

Summary

자동 번역

자동 번역

Denne protokol beskriver procedurerne for at fremkalde akut nyreskade (AKI) hos voksne zebrafisk ved hjælp af cisplatin som et nefrotoksisk middel. Vi detaljerede trin til at evaluere reproducerbarheden af teknikken og to teknikker til at analysere inflammation og celledød i henholdsvis nyrevævet, flowcytometri og TUNEL.

Tags

Acute Kidney InjuryCisplatinZebrafishNephrotoxicityInflammationCell DeathFlow CytometryTUNEL AssayChemotherapyRenal ProtectionRegeneration

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