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Xenopus胚胎细胞的粘膜异体器官：生成、培养和高分辨率实时成像
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JoVE 신문 생물학
Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
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Xenopus胚胎细胞的粘膜异体器官：生成、培养和高分辨率实时成像

DOI:
10.3791/61604-v

•

07:44 min

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July 28, 2020

•

,
1Center for Vascular Research,Institute for Basic Science

Chapters

  • 00:05Introduction
  • 00:57Isolation of Deep Ectoderm Cells
  • 03:09Generation of Mucociliary Epithelial Organoids
  • 04:29High-resolution Live Imaging of Developing Organoids
  • 05:23Results: Imaging of Developing Organoids
  • 06:53Conclusion

Summary

자동 번역

자동 번역

我们描述了一个简单的方案，从从 Xenopus laevis 胚胎分离的深层皮球细胞中开发粘膜上皮器官。多能祖细胞再生上皮杯细胞前体，并允许实时跟踪器官表面细胞过渡的开始和进展。

Tags

Keywords: Mucociliary EpitheliumXenopus Embryonic CellsOrganoid GenerationLive Cell ImagingCell TransitionsRapid ProtocolScalableReproducibleEmbryo CultureAnimal Cap ExcisionDeep Ectoderm Separation

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