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Chapters
- 00:05Introduction
- 00:45Cryosectioning and Tissue Section Storage
- 01:39Staining: Day 1
- 02:55Staining: Day 2
- 03:57Mounting
- 05:09Results: Representative Immunohistochemical Analysis of Free-Floating Tissue Sections
- 06:02Conclusion
The free-floating technique allows researchers to perform histological-based stainings including immunohistochemistry on fixed tissue sections to visualize biological structures, cell type, and protein expression and localization. This is an efficient and reliable histochemical technique that can be useful for investigating a multitude of tissues, such as brain, heart, and liver.
