JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Center for Genomic Medicine,Massachusetts General Hospital, 2Department of Psychiatry,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4Department of Psychiatry,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital
Chapters
- 00:04Introduction
- 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
- 01:46iPSC-Derived BMEC Subculture and Purification
- 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
- 04:09Efflux Transporter Activity and Analysis
- 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
- 07:18Conclusion
Ce protocole détaille une méthode adaptée pour dériver, étendre, et cryopréserver des cellules endothéliales microvasculaires de cerveau obtenues en différeciant les cellules souches pluripotentes induites par l’homme, et pour étudier des propriétés de barrière de cerveau de sang dans un modèle ex vivo.
