JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Université Côte d’Azur,Inserm UMR1065, C3M, Team “Cellular and Molecular Pathophysiology of Obesity”, Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine,Karolinska Institutet, Stockholm, Sweden, 3Université Côte d’Azur,Inserm UMR1065, C3M, Team “Haematometabolism in Diseases”, Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS),Université de Toulouse, CNRS, UPS, Toulouse, France
Chapters
- 00:04Introduction
- 00:35Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation
- 02:59Mouse and Human White Adipose Tissue Staining
- 05:28Mouse and Human White Adipose Tissue Clearing
- 06:173D Confocal Imaging
- 07:233D Adipose Tissue Image Quantitative Result Extraction
- 07:57Results: Representative Adipose Tissue Labeling and Analysis
- 09:34Conclusion
Qui, descriviamo un metodo semplice, economico e veloce per risolvere la struttura 3D del tessuto adiposo bianco e topo umano utilizzando una combinazione di marcatori per visualizzare vascolatura, nuclei, cellule immunitarie, neuroni e proteine del mantello di goccia lipidi mediante imaging fluorescente.
