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澳大利亚同步加速器串行晶体测量的利皮迪科注射协议

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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

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澳大利亚同步加速器串行晶体测量的利皮迪科注射协议

DOI:

10.3791/61650-v

•

07:28 min

•

September 23, 2020

•

Peter Berntsen, Rama Sharma, Michael Kusel, Brian Abbey, Connie Darmanin

¹Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science,La Trobe University, Melbourne, Australia, 3086, ²Kusel Design, Niddrie, Melbourne, Australia, 3042

Chapters

00:05Introduction
00:33High Viscous Medium Crystal Preparation
02:37Crystal Concentration Determination
03:28Injector Mounting and Control
04:14Sample Syringe Mounting
05:01Running the Injector
05:59Results: Representative Serial Crystallography Measurement
06:50Conclusion

Summary

자동 번역

该协议的目的是演示如何准备串行晶体学样本，以便于最近在澳大利亚同步加速器上委托的高粘度喷油器Lipidico上收集数据。

Tags

Keywords: Lipidico Injection Serial Crystallography Australian Synchrotron Protein Crystals Viscous Materials Glass Syringes Crystal Solution Centrifugal Force High-vacuum Silicone Grease Optical Microscope Crystal Concentration

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