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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron
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Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

Lipidico Injection Protocol for Serial Crystallography Measurements at the Australian Synchrotron

DOI:
10.3791/61650-v

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07:28 min

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September 23, 2020

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, , , ,
1Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science,La Trobe University, Melbourne, Australia, 3086, 2Kusel Design, Niddrie, Melbourne, Australia, 3042

Chapters

  • 00:05Introduction
  • 00:33High Viscous Medium Crystal Preparation
  • 02:37Crystal Concentration Determination
  • 03:28Injector Mounting and Control
  • 04:14Sample Syringe Mounting
  • 05:01Running the Injector
  • 05:59Results: Representative Serial Crystallography Measurement
  • 06:50Conclusion

Summary

자동 번역

자동 번역

The goal of this protocol is to demonstrate how to prepare serial crystallography samples for data collection on a high viscous injector, Lipidico, recently commissioned at the Australian synchrotron.

Tags

Lipidico InjectionSerial CrystallographyAustralian SynchrotronProtein CrystalsViscous MaterialsGlass SyringesCrystal SolutionCentrifugal ForceHigh-vacuum Silicone GreaseOptical MicroscopeCrystal Concentration

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