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1Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research,Università degli Studi di Milano, 2Department of Veterinary Medicine,University of Sassari, 3Department of Agricultural and Environmental Sciences – Production, Landscape, Agroenergy and Center for Stem Cell Research,Università degli Studi di Milano
Chapters
- 00:04Introduction
- 01:11Fibroblast Exposure to 5-aza-CR
- 02:28Fibroblast Encapsulation in PTFE Micro-Bioreactors
- 04:05Culture in PTFE Micro-Bioreactors of Epigenetically Erased Cells
- 05:00Results: Epigenetically Erased Cells Encapsulated in PTFE Micro-Bioreactors Show High Level and Long-term Maintenance of Pluripotency-Related Gene Expression
- 07:33Conclusion
We presenteren hier een methode die het gebruik van chemische epigenetische wissing combineert met mechanosensing-gerelateerde signalen om efficiënt pluripotente cellen van zoogdieren te genereren, zonder de noodzaak van gentransfectie of retrovirale vectoren. Deze strategie is daarom veelbelovend voor translationele geneeskunde en vertegenwoordigt een opmerkelijke vooruitgang in stamcelorganoïde technologie.
