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1Department of Medicine, Division of Infectious Diseases, Brigham and Women’s Hospital,Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women’s Hospital,Harvard Medical School
Chapters
- 00:04Introduction
- 01:02Preparation of Sterile Eye Swabs
- 01:48Workspace Setup
- 02:52Eye Swabbing
- 04:47Enrichment
- 06:02Results: Ocular Microbiome of C57BL/6 Mice
- 07:19Conclusion
Presented here is a protocol for the isolation and amplification of aerobic and facultative anaerobic mouse conjunctival commensal bacteria using a unique eye swab and culture-based enrichment step with subsequent identification by microbiological based methods and MALDI-TOF mass spectrometry.
