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在伤口反应期间斑马鱼中性粒细胞中趋化因子受体的实时成像

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses

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在伤口反应期间斑马鱼中性粒细胞中趋化因子受体的实时成像

DOI:

10.3791/61679-v

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06:48 min

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December 04, 2020

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Antonios Georgantzoglou, Caroline Coombs, Hugo Poplimont*¹, Hazel A. Walker*¹, Milka Sarris

¹Department of Physiology, Development and Neuroscience,University of Cambridge

Chapters

00:04Introduction
01:04Larval Ventral Fin Wounding and Live Imaging Preparation
02:22Live Confocal Imaging
03:03Zebrafish Neutrophil Receptor Internalization Quantification
04:36Results: Representative Differential CXCR1 and CXCR2 Wound Response Dynamics
06:12Conclusion

Summary

자동 번역

在这里，我们描述了对斑马鱼嗜中性粒细胞的化学引诱剂受体动力学进行实时成像和定量分析的方案。

Tags

Zebrafish Neutrophils Wound Response Chemokine Receptors Live Imaging In Vivo Inflammation Immune Response Transgenic Fin Wounding Confocal Microscopy Time-lapse Imaging Receptor Internalization

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