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1Laboratory of Molecular Gerontology, National Institute on Aging,National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology,Hunan University, 3Department of Molecular Biology and Genetics,Cornell University, 4Department of Cellular and Molecular Medicine,University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
Chapters
- 00:04Introduction
- 00:51Cell Preparation
- 03:58Proximity Ligation Assay (PLA)
- 06:20Results: Representative Replisome Encounter Visualization
- 07:52Conclusion
While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.
