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1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2DOE-Joint Genome Institute,Lawrence Berkeley Laboratory, 3Kearney Agricultural Research and Extension Center,University of California Agriculture and Natural Resources, 4West Side Research and Extension Center,University of California, 5Department of Plant Sciences,University of California, Davis, 6Department of Plant and Microbial Biology,University of California, Berkeley
Chapters
- 00:05Introduction
- 00:40Nuclei Isolation from Sorghum Leaf Sample
- 02:13Isolation of Nuclei from Remaining Cytoplasmic Contaminants
- 03:05Nuclei Lysis and Histone Extraction
- 04:39Results: Qualitative & Quantitative Analysis of the Identified Histones
- 06:36Conclusion
Le protocole a été développé pour extraire efficacement les histones intactes des matériaux de feuilles de sorgho pour le profilage des modifications post-translationnelles de l’histone qui peuvent servir de marqueurs épigénétiques potentiels pour aider à l’ingénierie des cultures résistantes à la sécheresse.
