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Chapters
- 00:04Introduction
- 00:39Light Sheet Fluorescence Microscope Calibration
- 02:37Sodium Hypochlorite-Based Dechorionation
- 03:48Embryo Mounting
- 04:51Comparative Live Imaging
- 06:02Imaged Embryo Retrieval and Cultivation
- 06:52Results: Representative Drosophila and Tribolium Fluorescence Live Imaging
- 07:56Conclusion
Light sheet-based fluorescence microscopy is the most valuable tool in developmental biology. A major issue in comparative studies is ambient variance. Our protocol describes an experimental framework for simultaneous live imaging of multiple specimens and, therefore, addresses this issue pro-actively.
