Journal
/
/
Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (Apis mellifera)
DOI:

06:41 min

August 26, 2020

, ,

Chapters

  • 00:04Introduction
  • 00:41Larval Self-Removal
  • 02:08Pupal Virus Injection
  • 03:19Viral Feeding Bioassay
  • 04:58Results: Representative Virus Load and Survival Curve Analyses
  • 05:55Conclusion

Summary

자동 번역

Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay.

Related Videos

Read Article