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Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
JoVE 신문
생체공학
Author Produced
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JoVE 신문 생체공학
Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
DOI:

14:48 min

April 17, 2021

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Chapters

  • 00:00Introduction
  • 02:23Microfluidic Chip Design
  • 04:33Chip Fabrication and Operation
  • 05:32Microfluidic Chip Production using Soft Lithograph
  • 07:03Chip Operation
  • 07:59Generation of Dynamic Inputs in Cellular Microenviroments
  • 08:43Setup for High Throughput Live-cell Imaging
  • 10:44Automatic Data Analysis
  • 11:42Discussion

Summary

자동 번역

We present a microfluidic system for high throughput studies on complex life machinery, which consists of 1500 culture units, an array of enhanced peristaltic pumps and an on-site mixing modulus. The microfluidic chip allows for the analysis of the highly complex and dynamic micro-environmental conditions in vivo.

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