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Quantifying the Effects of Antimicrobials on In vitro Biofilm Architecture using COMSTAT Software
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Chapters
- 00:00Introduction
- 00:27Confocal Microscopy Imaging and COMSTAT Analysis
- 04:21Results: Quantification of Antimicrobial Effects on In Vitro Biofilm Architecture
- 05:49Conclusion
Antimicrobial-induced changes to Pseudomonas aeruginosa biofilm architecture differ among clinical isolates cultured from patients with cystic fibrosis and chronic pulmonary infection. Following confocal microscopy, COMSTAT software can be utilized to quantify variations in biofilm architecture (e.g., surface area, thickness, biomass) for individual isolates to assess the efficacy of anti-infective agents.
