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Kapillær elektroforese massespektrometri tilgange til karakterisering af protein og metabolit Corona erhvervet af nanomaterialer
JoVE 신문
화학
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JoVE 신문 화학
Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
DOI:

07:54 min

October 27, 2020

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Chapters

  • 00:04Introduction
  • 00:30Biomolecular Corona Formation and Protein Corona Isolation
  • 02:52Metabolite Corona Isolation
  • 03:50Preparation of CESI Capillaries and CE-MS for Protein Corona Analysis
  • 05:11Preparation of CESI Capillaries and CESI-MS for Metabolite Corona Analysis
  • 06:27Results: Mass Spectral Analysis of the Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
  • 07:26Conclusion

Summary

자동 번역

Her præsenterer vi en protokol til at karakterisere den komplette biomolekylære korona, proteiner og metabolitter, erhvervet af nanomaterialer fra biofluider ved hjælp af en kapillær elektroforese – massespektrometri tilgang.

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